Induced Pluripotent Stem Cells (iPSCs) — economic tools for cell reprogramming

iPS cells are a type of pluripotent stem cell artificially derived, and often referred to as programmed, from adult somatic cells using the expression of certain genes in culture. Using adult somatic cells, they have been reprogrammed to an embryonic stem cell-like state by being forced to express genes and factors important for maintaining undifferentiated embryonic stem cells.

GeneCopoeia has several tools and related reagents that are useful to the stem cell scientist interested to perform reprogramming trials. More detailed information is found on the product-specific web page or send an inquiry to: inquiry@genecopoeia.com.

Lentifect™ Ready-to-Transduce Lentiviral Particles — both crude and purified for all iPSC genes

GeneCopoeia Lentifect™ lentiviral particles are produced from standardized protocols using high-purity plasmid DNA preparations and EndoFectin™ Lenti transfection and TiterBoost™ reagents ensuring production of the highest-titers possible of crude or purified particles ever achieved. Ready-for-transduction, lentiviral preps from GeneCopoeia offer a cost-effective choice for busy labs.

Purified lentiviral particles for iPSC genes

Buy Catalog# Product Description* LPP-CMYC-LV105-025 c-Myc Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) ≈ 8 x 109 copies/ml lentiviral particles ready for transduction, 25 µl LPP-KLF4-LV105-025 Klf4 Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) ≈ 1 x 109 copies/ml lentiviral particles ready for transduction, 25 µl LPP-NEG-LV105-025 Negative Control Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) ≈ 3.3 x 1011 copies/ml control lentiviral particles ready for transduction, 25 µl LPP-OCT4-LV105-025 Oct4 Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) ≈ 4.5 x 109 copies/ml lentiviral particles ready for transduction, 25 µl LPP-SOX2-LV105-025 Sox2 Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) ≈ 3.4 x 107 copies/ml lentiviral particles ready for transduction, 25 µl

*Titer determined by quantitative PCR on crude lentiviral supernatant.

Crude lentiviral particles for iPSC genes

Buy Catalog# Product Description* LP-CMYC-LV105-0200 c-Myc Lentifect™ Lentiviral Particles (200 µl x 1 vial) ≈ 8 x 108 TU/ml lentiviral particles ready for transduction, 200 µl LP-CMYC-LV105-0205 c-Myc Lentifect™ Lentiviral Particles (200 µl x 5 vials) ≈ 8 x 108 TU/ml lentiviral particles ready for transduction, 5 x 200 µl LP-CMYC-LV105-1000 c-Myc Lentifect™ Lentiviral Particles (1 ml x 1 vial) ≈ 8 x 108 TU/ml lentiviral particles ready for transduction, 1 x 1 ml LP-KLF4-LV105-0200 Klf4 Lentifect™ Lentiviral Particles (200 µl x 1 vial) ≈ 1 x 108 TU/ml lentiviral particles ready for transduction, 200 µl LP-KLF4-LV105-0205 Klf4 Lentifect™ Lentiviral Particles (200 µl x 5 vials) ≈ 1 x 108 TU/ml lentiviral particles ready for transduction, 5 x 200 µl LP-KLF4-LV105-1000 Klf4 Lentifect™ Lentiviral Particles (1 ml x 1 vial) ≈ 1 x 108 TU/ml lentiviral particles ready for transduction, 1 x 1 ml LP-NEG-LV105-0200 Negative Control Lentifect™ Lentiviral Particles (200 µl x 1 vial) ≈ 3.3 x 1010 copies/ml control lentiviral particles ready for transduction, 200 µl LP-NEG-LV105-0205 Negative Control Lentifect™ Lentiviral Particles (200 µl x 5 vial) ≈ 3.3 x 1010 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl LP-NEG-LV105-1000 Negative Control Lentifect™ Lentiviral Particles (1 ml x 1 vial) ≈ 3.3 x 1010 copies/ml control lentiviral particles ready for transduction, 1 ml LP-OCT4-LV105-0200 Oct4 Lentifect™ Lentiviral Particles (200 µl x 1 vial) ≈ 4.5 x 108 TU/ml lentiviral particles ready for transduction, 200 µl LP-OCT4-LV105-0205 Oct4 Lentifect™ Lentiviral Particles (200 µl x 5 vials) ≈ 4.5 x 108 TU/ml lentiviral particles ready for transduction, 5 x 200 µl LP-OCT4-LV105-1000 Oct4 Lentifect™ Lentiviral Particles (1 ml x 1 vial) ≈ 4.5 x 108 TU/ml lentiviral particles ready for transduction, 1 x 1 ml LP-SOX2-LV105-0200 Sox2 Lentifect™ Lentiviral Particles (200 µl x 1 vial) ≈ 3.4 x 107 TU/ml lentiviral particles ready for transduction, 200 µl LP-SOX2-LV105-0205 Sox2 Lentifect™ Lentiviral Particles (200 µl x 5 vials) ≈ 3.4 x 107 TU/ml lentiviral particles ready for transduction, 5 x 200 µl LP-SOX2-LV105-1000 Sox2 Lentifect™ Lentiviral Particles (1 ml x 1 vial) ≈ 3.4 x 107 TU/ml lentiviral particles ready for transduction, 1 x 1 ml

Learn more about ready-to-transduce lentiviral particles.

OmicsLink™ Expression-Ready ORF cDNA Clones — wide selection of lentiviral vectors and tags

The OmicsLink™ expression-ready clone family is the most extensive and complete source of standard and specialized plasmid constructs anywhere. GeneCopoeia has several genes used in reprogramming available in many vectors with a variety of fusion tags. If your clone search doesn't find what you are looking for, send us an email at inquiry@genecopoeia.com.

Click on product ID to view the vector options available for these genes

Learn more about expression-ready ORF cDNA clones.

OmicsLink™ Expression-Ready shRNA Clones — stable and guaranteed knockdown

For every gene you want to down regulate through RNA interference, GeneCopoeia uses a proprietary algorithm to carefully prepare four sequence-specific shRNA constructs. These constructs are available in lentiviral or non-viral vectors with a choice of eGFP or mCherry reporter gene. GeneCopoeia guarantee that at least one of the four constructs will give you 70% knockdown when tested with qRT-PCR or we will replace it with a new one.

Click on product ID to view the vector options available for these genes

Learn more about expression-ready shRNA clone collection.

GeneCopoeia also offers validated qRT-PCR primers for iPSC genes.