miExpress™ Precursor microRNA (miRNA) Expression Clones —
study the regulatory and functional effect of miRNAs on corresponding genes and proteins
GeneCopoeia offers precursor microRNA expression constructs in a lentiviral (FIV-based) and non-viral vector based systems with eGFP reporter gene. All known miRNAs in miRBase are available from GeneCopoeia.
- 750 human
- 450 mouse
- 270 rat
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GeneCopoeia miRNA constructs are made with proprietary, state-of-the-art technology, are fully sequenced and come with a guarantee ensuring the integrity of critical regions. For you, that means greater confidence and accurate results faster.
Choose the right vector backbone for your experiments
| 1. |
FIV-based lentiviral microRNA
expression vector backbone - $396 |
2. |
Non-viral microRNA
expression vector backbone - $247 |
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Features and benefits of precursor miRNA expression clone collections
| Versatile |
Efficient infection-based transduction of miRNAs into non-dividing and otherwise difficult-to-transfect target cells as well as conventional transfection-based transduction into dividing cells. |
| Efficient |
Stable cell-line selection marker (neomycin) enables regulation studies of both long-term and transient expression. |
| Comprehensive |
Vector-plasmid transfection and virus-infection transduction efficiencies are monitored with the eGFP reporter protein. |
| High Quality |
Fully-sequenced expression cassettes of all miRNA expression constructs. |
Figure 1. Lentiviral vector based precursor miRNA expression clones and the mechanism of vector mediated miRNA gene regulation
Overview of miRNAs and miExpress precursor constructs
MicroRNAs (miRNAs) are small, non-coding single stranded RNA molecules found in eukaryotic organisms. They are highly conserved and usually 21-23 nucleotides in length. miRNAs are involved in almost all cellular functions and regulate gene expression by binding to the 3’ untranslated regions (3’ UTRs) of targeted mRNAs. Irregularities in miRNA-regulated gene expression have been found to be associated with cancers, cardiovascular disorders and a variety of other diseases.
miExpress precursor miRNA constructs includes an RNA polymerase III type promoter (H1) which drives transcription of hairpin precursor miRNAs (approximately 150 nucleotides). These precursor miRNAs are then processed to generate mature miRNAs by RNAi enzymes. The eGFP tracking reporter gene is co-expressed with neomycin selection gene under the control of CMV promoter and bicistronic internal ribosome entry site (IRES) element, providing a convenient method for monitoring miRNA expression, transfection and transduction efficiencies.
RISC-miRNA complexes specifically regulate gene expression by binding to the 3’ UTR of mRNAs of target genes resulting in either translation suppression or mRNA cleavage, degradation, and translation inhibition.
Complete your functional analysis studies with
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Publication
Kim, H W et al. (2009) Ischemic Preconditioning Augments Survival of Stem Cells via miR-210 Expression by Targeting Caspase-8-associated Protein 2*. The Journal of Biological Chemistry 284(48):33161–33168. [pEZX-miR-210 and luciferase reporter construct containing the 3-UTR of Casp8ap2]
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