Protein Subcellular Localization & Validated Organelle Markers 
The study of protein subcellular localization is necessary to elucidate protein function. To facilitate protein localization studies inside cells, organelles and tissues, GeneCopoeia offers multiple sets of expression-ready ORF cDNA constructs. These constructs consist of full-length mouse and human cDNA ORFs fused with various tags including eGFP, mCherry, SNAP-Tag™ and HaloTag®.
Fully-sequenced, expression-ready GeneCopoeia ORF cDNA tagged plasmids enable many studies including:
- Subcellular protein localization in live and fixed cells
- Monitoring of multiple proteins trafficking in one assay
- Protein detection, tracking and characterization
- Organelle morphology without antibodies
- Co-localization (see figure 1)
Co-localization of two proteins in the same cell using OmicsLink™ ORF expression clones
Figure 1. TOMM20 (translocase of outer mitochondrial membrane 20 homolog) is located in the mitochondria. LEF1 (Lymphoid enhancer-binding factor 1) is located in the nucleus. Using standard cell and transfection techniques, cells were co-transfected with two plasmids; EX-G0283-M26 (encoding the TOMM20 gene fused with SNAP-Tag™ ) and EX-W0594-M50 (encoding the LEF1 gene fused with HaloTag®). Twenty-four hours after transfection, the cells were labeled with HaloTag® TMR ligand and SNAP Cell BG505 ligand according to the manufacturer's instructions. The nuclei were stained with DAPI and the subcellular structures of the cells were imaged using fluorescent microscopy: (1) cells labeled with Halo-TMA ligand (red); (2) cells labeled with SNAP Cell BG505 ligand (green); (3) overlay of 1 and 2; (4) cells stained with DAPI; and (5) overlay of 1, 2 and 4.
Validated organelle markers and predicted subcellular localization genes
Click on each organelle name for gene list or Browse all validated organelle markers and predicted subcellular localization genes.
Human full-length ORF expression clones for studying protein localization
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